Name: Tris (hydroxymethyl) methyl aminomethane THAM

Other names: Tris base; 2-Amino-2- (hydroxymethyl) -1,3-propanediol; THAM; Trometamol

CAS : 77-86-1

Molecular formula: C4H11NO3

Linear molecular formula: NH2C (CH2OH) 3

Molecular weight: 121.14

MDL number: MFCD00004679

Beilstein: 741883

EC : 201-064-4

Description: white crystalline particles

Physical parameter

pKa: 8.1

pH use range: 7-9

Melting point: 168-172 ° C

Boiling point: 219-220 ° C / 10mmHg (lit.)

Usage:

Tris buffer is not only widely used as a solvent for nucleic acids and proteins, it also has many important uses. Tris is used for protein crystal growth at different pH conditions. [3] The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of C. elegans lamin. [4] Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators, and some drugs. Tris is also used as a titration standard.